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Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

Categories Sequencing Grade Trypsin
Brand Name: YaxinBio
Model Number: SRCT03
Certification: ISO9001
Place of Origin: China
MOQ: 100ug
Payment Terms: T/T
Packaging Details: dry ice
Price: 158$/100ug
Supply Ability: 1000g per week
Delivery Time: 7 days
Source: E.Coli
Purity: NLT 95% by HPLC analysis.
Physical form: Lyophilized powder
Specific activity: NLT 1500 USP units/mg pro
Contaminant activity: No carboxypeptidase A, or other proteases contaminant.
CAS: 9004-07-3
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    Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

    Sequencing Grade Chymotrypsin for Digestion Reactions In-solution


    Descirption


    Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

    The sequencing grade human chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

    CAS: 9004-07-3
    EC: 3.4.21.1

    Source: human chymotrypsin, expressed in E. Coli.


    Main Features


    Source

    E. Coli

    M.W.

    26,950 Da

    Purity

    ≥ 98 % by HPLC.

    Format

    Lyophilized

    Specific activity

    ≥ 3000 units/mg pro


    Application


    1) Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

    2) This sequencing grade enzyme can be used alone or in combination with other proteases to produce.


    Recommend Usage


    For peptide digestion, use a ratio (w/w) of approximately 1:20-1:100 for chymotrypsin:peptide. Perform peptide digests in 40 mM Tris-HCl pH7.8 containing 5 mM CaCl2, at 30 °C. Self digestion may occur if temperatures above 37°C.


    Storage Instructions


    Recombinant Chymotrypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

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